Embryology Research Today is a free monthly online journal that collates and summarizes the latest research about Embryology, including details on stem cells, reproduction, transplants, cloning.

Effect of rescuing donated immature human oocytes derived after FSH/hCG stimulation following in vitro culture with or without Follicular Fluid Meiosis Activating Sterol (FF-MAS)-an embryo chromosomal and morphological analysis.

Lundin K, Ziebe S, Bergh C, Loft A, Selleskog U, Nilsson L, Grøndahl C,

Department of Obstetrics and Gynecology, Reproductive Medicine, Sahlgrenska University Hospital, Göteborg, Sweden, kersti.lundin@vgregion.se.

Purpose: Studies in mice and humans have shown that Follicular Fluid - Meiosis Activating Sterol (FF-MAS) induces meiotic maturation of immature oocytes in vitro. A multicenter, prospectively randomised study evaluated chromosomal status of embryos from FSH/hCG primed human immature oocytes, cultured with or without FF-MAS. Methods: Denuded immature oocytes (n=365) were randomly allocated into inert control, FF-MAS 5 muM or 20 muM. Seventy +/-2 hours after ICSI on matured oocytes, all cleaved embryos were fixed for fluorescence in situ hybridisation analysis. Results: Only 15% of oocytes resulted in cleaved embryos. GV oocytes matured at significantly lower rates (14% and 7%) in the two FF-MAS groups compared to the inert control group (47%). High rates of chromosomal abnormalities were found in all groups. Conclusion: Immature oocytes showed poor development with high rates of embryo chromosomal abnormalities. Exposure to FF-MAS in the concentrations, duration and/or formulation used in this study did not improve the results.

Published 11 January 2007 in J Assist Reprod Genet.
Full-text of this article is available online (may require subscription).

© 2005-2008 Embryology Research Today. All Rights Reserved.