Embryology Research Today is a free monthly online journal that collates and summarizes the latest research about Embryology, including details on stem cells, reproduction, transplants, cloning.

In vivo evaluation of indolyl glyoxamides in the phenotypic sea urchin embryo assay.

Semenova MN, Kiselyov AS, Titov IY, Raihstat MM, Molodtsov M, Grishchuk E, Spiridonov I, Semenov VV

Institute of Developmental Biology, Russian Academy of Sciences, 26 Vavilov Str, 119334 Moscow, Russia. ms@chemical-block.com

We have devised a 'one-pot' phenotypic in vivo assay for the rapid evaluation of potential tubulin inhibitors using the sea urchin embryo model. An effect of a small molecule on two specific developmental stages of sea urchin embryo, namely: (i) fertilized egg test for antimitotic activity and (ii) behavioral monitoring of a free-swimming blastulae for changes in the embryo swimming pattern could be quantified by a threshold concentration resulting in respective abnormalities. Derivatives of the clinical candidate D-24851 featured good correlation between activity in tubulin polymerization assay and our in vivo data. Importantly, we demonstrated that in these series, the N-substitution of indole is non-essential to attain profound in vitro and cellular effects.

Published 23 November 2007 in Chem Biol Drug Des, 70(6): 485-90.
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