Embryology Research Today is a free monthly online journal that collates and summarizes the latest research about Embryology, including details on stem cells, reproduction, transplants, cloning. | ||||||||
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Effects of androgen on embryo implantation in the mouse delayed-implantation model.Diao HL, Su RW, Tan HN, Li SJ, Lei W, Deng WB, Yang ZM College of Life Science, Xiamen University, Xiamen, China; College of Life Science, Northeast Agricultural University, Harbin, China. OBJECTIVE: To examine the effects of androgen on implantation and decidualization in the mouse delayed-implantation model. DESIGN: Experimental animal study. SETTING: University research laboratory. ANIMAL(S): Sexually mature female mice (Kunming White strain). INTERVENTION(S): Delayed and activated implantation; pseudopregnancy; embryo transfer (ET); E(2) assay; inhibitor. MAIN OUTCOME MEASURE(S): Effects of androgen on embryo implantation were determined by treating the mice under delayed implantation with different doses of testosterone propionate (TP); the effects of androgen on the expression of implantation-related genes were examined by in situ hybridization. RESULT(S): Delayed implantation could be initiated by TP. Dihydrotestosterone was also able to initiate implantation in the delayed-implantation model. The implantation window could be maintained for at least 48 hours by 5 mg TP per mouse. Prostaglandin endoperoxide synthase 2 (Ptgs2) and microsomal prostaglandin E synthase (mPtges) were aberrantly expressed in mouse uterus at implantation sites after delayed implantation was activated by high doses of TP. CONCLUSION(S): A low dose of TP led to a delay in embryo implantation, but a high dose caused aberrant expression of both Ptgs2 and mPtges at the implantation site. It is possible that high doses of TP may disturb peri-implantation development or may be involved in early pregnancy loss by disturbing the uterine prostaglandin system. Published 6 December 2007 in Fertil Steril.
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