Embryology Research Today is a free monthly online journal that collates and summarizes the latest research about Embryology, including details on stem cells, reproduction, transplants, cloning. | ||||||||
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Lysed cell removal promotes frozen-thawed embryo development.Elliott TA, Colturato LF, Taylor TH, Wright G, Kort HI, Nagy ZP Reproductive Biology Associates, Atlanta, Georgia 30342, USA. Thomas@ivf.net OBJECTIVE: To develop a mouse model to investigate the possible causes for increased success rates when lysed cells are removed from thawed embryos. DESIGN: Experimental study. SETTING: Clinical IVF laboratory. INTERVENTION(S): Assisted hatching, cell lysis, and removal of lysed cells. MAIN OUTCOME MEASURE(S): Embryonic growth rate and morphology. RESULT(S): The mouse embryos were divided into three groups; control (no cell lysis), group 1 (cell lysis and removal), and group 2 (cell lysis only). There was no significant difference in the initial number of blastomeres in each group or the number of cells lysed artificially in groups 1 and 2. The rate of embryonic development showed a significant delay in group 2 (7.97 +/- 4.92; control, 10.42 +/- 8.18; group 1, 5.74 +/-4.42; group 2). The embryo morphology on day 4 was significantly improved in group 1 and the control group when compared with group 2. CONCLUSION(S): Mouse embryos with artificially lysed cells after thawing had poorer developmental quality and growth rates compared with control embryos. However, removal of lysed cells restored the embryo's developmental potential to that of the control. Cell number and morphology was also significantly improved compared with embryos without lysed cell removal. These findings are consistent with human embryo development after thawing when lysed cells are present and thus mechanical lysis seems to be an appropriate method by which to further study frozen-thawed lysed cell removal. Published 4 June 2007 in Fertil Steril, 87(6): 1444-9.
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